Journal: The Journal of Experimental Medicine

Article Title: Piezo1 and G q /G 11 promote endothelial inflammation depending on flow pattern and integrin activation

doi: 10.1084/jem.20180483

Figure Lengend Snippet: Integrins are differentially activated by different flow patterns via Piezo1 and G q /G 11 . (A–C) HUAECs were transfected with scrambled siRNA (control) or siRNA directed against ITGA5 and ITGAV (A) or against Gα q and Gα 11 (Gα q/11 ; B and C) and were exposed to laminar or disturbed flow as described in . Activation of integrin signaling was determined by immunoblotting for phosphorylated focal adhesion kinase (pFAK, Y397; A) or by immunoprecipitation of activated α5 integrin (B and C). P65 activation was determined by anti–phospho-P65 (S536) antibodies (A). Bar diagrams show densitometric evaluation of immunoblots (quantification of three to five independent experiments). (D) Ldlr-KO mice without or with endothelium-specific Gα q /Gα 11 deficiency (Ldlr-KO;EC-Gα q/11 -KO) endothelium-specific Piezo1-deficiency (Ldlr-KO;EC-Piezo1-KO) were fed a high-fat diet for 4 wk ( n = 4–6 mice per genotype). Cross sections of the inner and outer curvatures of aortic arches were immunostained with antibodies against activated α5 integrin (SNAKA51; green), against the endothelial marker CD31 (red) or with DAPI (blue). Bar diagrams show percentage of area stained by anti-activated α5 integrin antibody of total endothelial cell area defined by staining by anti-CD31 antibody (based on analysis of at least five sections each from at least four different animals). Bars, 25 µm. Data represent mean ± SEM; *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001 (two-tailed Student’s t test).

Article Snippet: Cells were then incubated in flow chambers with primary antibody directed against activated integrin α5, SNAKA51 (Novus Biologicals, NBP2-50146), overnight at 4°C (1:100 dilution).

Techniques: Transfection, Control, Activation Assay, Western Blot, Immunoprecipitation, Marker, Staining, Two Tailed Test

Journal: Cells

Article Title: Long-Term Low-Dose Delta-9-Tetrahydrocannbinol (THC) Administration to Simian Immunodeficiency Virus (SIV) Infected Rhesus Macaques Stimulates the Release of Bioactive Blood Extracellular Vesicles (EVs) that Induce Divergent Structural Adaptations and Signaling Cues

doi: 10.3390/cells9102243

Figure Lengend Snippet: Chemical reagents and instrument used in this study.

Article Snippet: Integrin α5 (D7B7G) , Cell Signaling , Beverly, MA, USA , 98204.

Techniques: Staining, Membrane, Microscopy, Imaging